Source:
Chicken Gizzard

 

Applications:
Protein standard in 1D and 2D SDS gelelectrophoresis
Immunoassays Immunization

 

Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.

 

Formulation:
The protein was lyophilized from a 1mg/ml solution containing 20 mM Tris / acetate buffer pH 7.6, 0.1mM EDTA, 2 mM DTT and 20 mM NaCl. Each mg Filamin contains 550mg urea.

 

Solubility:
It is recommended to reconstitute the lyophilized Filamin in sterile 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability:
Lyophilized Filamin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Filamin should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

 

Purity:
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.