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Monoclonal anti-GST antibody (clone 1E5)
use IgG 2b, ?
Cat. No. AGT0501 Size : 100 µl
Cat. No. AGT0515 Size : 50 µl |
Immunogen: Recombinant Glutathione S transferase (GST) purified from E. coli
Isotype: Mouse IgG2b heavy chain and ? light chain
Clone: Anti-GST mAb, clone 1E5, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant GST protein.
Description: Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GST proteins can be purified by immobilized glutathione affinity chromatography. Many recombinant proteins have been engineered with GST tags to facilitate the detection, isolation and purification of these proteins. Monoclonal antibody reacting specifically with GST may be useful in various immunotechnique, to identify the expression of a GST fusion protein in bacteria, bacterial lysates or cells and tissues transfected with a GST fusion protein expressing vectors
Concentration: 1mg/ml
Form: Liquid. In Phosphate-Buffered Saline(pH 7.4) with 0.02% thimerosal
Preparation and Storage: The antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. Can be stored at 4? for up to one month, but store at -20? for long term storage. Avoid repeated freezing and thawing cycles.
Usage: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should titrate the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:2,000.
Recommended starting dilution is 1:1,000.
Western blot analysis
Recombinant GST (28kDa) and GST-fusion protein (61kDa) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-GST antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Arrows indicate GST and GST-fusion proteins(20ng).
Application: ELISA, Western blot
General references : Smith, D., et al., Proc. Natl. Acad. Sci. USA, 83, 8703 (1986).
Cartwright, G., et al., J. Immunol. Meth., 179, 31 (1995).
Hibma, M., et al., Nucl. Ac. Res., 22, 3806 (1994).
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