International  +32 (0) 2 732 56 88  +32 (0) 2 732 44 14
France  01 43 25 01 50  01 43 25 01 60
Italy  02 36 00 65 93  02 36 00 65 94
Japan  +81 78 386 0860  +81 78 306 0296
 
 
 
OPYM-10 - Ultra filter unit for serum pretreatment (MW cut off 10kDa)
Product details
Product number OPYM-10
Product name Ultra filter unit for serum pretreatment (MW cut off 10kDa)
Quantity 1 unit
Supplier Gentaur
Ultra filter unit for serum pretreatment (MW cut off 10kDa)

The lab standard for sub-microliter volumes
- Solute recoveries typically >95%, ideal for dilute (ng/mL to µg/mL range) protein solutions
- Patented deadstop for reproducible concentrate volumes
- Inverted spin method of concentrate retrieval maximizes recovery
- Low-binding Ultracel membrane
- Color coding indicates MW cut-off
- Convenient sample storage of filtrate or concentrated sample in standard microfuge collection tube
- Use in standard 1.5 mL tube fixed-angle rotors


Applications
- Concentrating and purifying proteins, antibodies and nucleic acids (alternative to EtOH precipitation)
- Desalting and buffer exchange
- Removal of primers, linkers and unincorporated label


Typical Protein Recovery

   
Retentate Recovery (%)
Solute (Concentration)
Nominal MW
3
10
30
50
100
Bovine IgG Fraction II (1 mg/mL)
156,000
95
95
95
95
95
Bovine Serum Albumin (1 mg/mL)
67,000
95
95
95
90
50
Ovalbumin (1 mg/mL)
45,000
95
95
95
90
10
a-Chymotrypsinogen (1 mg/mL)
25,000
95
95
95
80
—
Cytochrome c (0.25 mg/mL)
12,400
95
95
90
10
—


Specifications   

Volumes
Maximum Initial Sample Volume, mL
0.5
Typical Final Concentrate Volume, µL
5–15
Centrifugal Force*
Fixed-angle microfuge rotor w/maximum 14,000 x g
Materials
Membrane
Ultracel regenerated cellulose
Device
Sample reservoir: polypropylene; Sample vial: polycarbonate
Membrane Support Base
Acetal
O-ring
Medical grade silicone rubber
Dimensions
Active Membrane Area, cm2
0.32
Diameter, mm
12.3
Length, in concentration mode, mm
45
*500 x g for DNA/RNA applications with Microcon YM-100 Filter Unit
Price
EUR10
USD12
GBP8
DKK75
JPY1.646
PLZ34
SEK93
NOK79
Buy or request info
  
Selected products
Europe - Gentaur Molecular Products bvba, Av. de l' Arme 68, B-1040 Brussels Tel:  +32 2 732 56 88 Fax. : +32 2 732 44 14
France 9, rue Lagrange 75005 Paris Tel:  01 43 25 01 50 Fax:  01 43 25 01 60
Italy Tel:  02 36 00 65 93 Fax: 02 36 00 65 94
Japan, Minaatojimaminami-manchi, Chuo-ku, Kobe 065-0047 Tel: +81 78 386 0860Fax: +81 78 306 0296