Features
          • Dramatically improves specificity and yield
          • Compatible with all commercially available thermostable DNA polymerases
          • Ideal for difficult templates
          • Reduces smearing and background

Applications
         • DNA Polymerase reactions where specificity is critical
         • Enhancing the performance and specificity of any thermostable DNA polymerase

Description
PolyMate is a special 2x additive for use in reactions involving any thermostable DNA polymerase, and is designed to dramatically improve reaction specificity. PolyMate provides an optimized composition of reagents, and is ideally suited to dirty/difficult templates with GC or AT rich DNA, repetitive sequences or sequences with a high level of secondary structure.
PolyMate acts as a melting agent by allowing the DNA polymerase and oligonucleotides greater access to the template DNA. PolyMate does not contain magnesium, dNTPs, or buffer components. In some cases it may be necessary to optimize the Magnesium concentration.

Note: PolyMate should not be used in combination with any other additives for polymerase reactions. PolyMate is a revised version of Bioline 5x Hi-Spec Additive (BIO-37032).

Directions for use:
Compose the reaction mix, containing buffer, dNTPs, Mg2+, template DNA, primers, DNA polymerase.
Add 2x PolyMate at the volume of half of the final volume of the reaction (e.g. 25ul per 50ul final volume, etc).
Add ddH2O up to the final volume and mix with pipetting.

Batch No: See vial
Storage Conditions: PolyMate Additive can be stored for 6 months at -20°C.
Shipping Conditions: Shipped on Dry or Blue Ice