1. Background
Benzyl penicillin (BP) is a broadly applied antibiotic, which once played very important role in preventing and curing animal diseases. For it causes anaphylactic reaction and resistance, in EU ,US and China, it is being restricted. The common instrumental analysis of this drug is limited because of the complicated operation and high expense, while this kit is a new product based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one detection, so it can considerably minimize operation error and work intensity.
2. Test Principle
This kit is based on indirect-competitive ELISA technology. The microtiter wells are coated with coupling antigen. BP residue in the sample competes with the antigen coated on the microtiter plate for the antibody. After the addition of enzyme labeled anti-antibody, TMB substrate is used to show the color. Absorbance of the sample is negatively related to the BP reside in it, after comparing with the Standard Curve, multiplied by the dilution factor, BP residue quantity in the sample can be calculated.
3. Applications
This kit can be used in quantitative and qualitative analysis of BP residue in animal tissue (muscle, liver, shrimp and fish), honey and milk,etc.
4. Cross-reactions
Benzyl penicillin (BP)…………………………… 100%
ampicillin ……………………………… 0.7%
cloxacillin ……………………………… 0.2%
dicloxacillin …………………………… 0.1%
amoxicillin …………………<0.1%
Ceftiofur …………………………………<0.1%
5. Materials Required
5.1 Equipments