This test kit is based on the competitive enzyme immunoassay for the detection of Ractopamine in the pork. The coupling antigen is pre-coated on the micro-well stripes. The Ractopamine in the testing sample competes with the coupling antigens pre-coated on the micro-well stripes for the antibodies against Ractopamine. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Ractopamine in it. This value is compared to the standard curve and the content of the corresponding  Ractopamine is subsequently obtained.

Sensitivity : 0.5 ppb
Detection limit
Muscle 1ppb
Liver 2ppb
Urine 1ppb
Admixture 25ppb
Contrated material ,premix material 75ppb
Recovery rate
Urine 85±10%
Muscle and liver 80±10%
Cross-reactions
Ractopamine 100%
Dobutamine 10%
Clenbuterol <0.1%
Salbutamol <0.1%