1. Add
2X volume of Dye Wash Buffer to sequencing reaction
product.
(If your product volume less then 20 µl, adjust final
volume to 20
µl with water). Mix gently by pipetting several times.
2. Place the
Dye Remove Plate on the vacuum manifold.
3. Transfer diluted sample from Step 1 into the bottom of each well.
4. Set the vacuum to 25" Hg and turn on the vacuum pressure for 5-10
minutes until each well was empty.
5. Turn off the vacuum.
6. Add
100 µl Dye Wash buffer to each well on the plate.
7. Turn on the vacuum pressure for 5-10 minutes or until each well was
empty.
8. Turn off the vacuum and remove the plate from the manifold.
9. Press the plate on an absorbent material (tissue paper) to blot out the
excess liquid from the bottom of the plate.
10. Place the Dye Remove Plate on the vacuum manifold and turn on the
vacuum pressure for 5 minutes.
11. Add
20-30 µl of sequencing loading buffer or deionized water
into central of the membrane. Pipet up and down 5-10 times to elute
the sequencing product.
12. Transfer eluted products to a new 96 well
0.35ml collection plate.
Centrifuge Protocol
1. Add
2X volume of Dye Wash Buffer to sequencing reaction
product.
(If your product volume less then 20 µl, adjust final
volume to 20
µl with water). Mix gently by pipetting several times.
2. Place the
Dye Remove Plate on the waste collection plate( not
provide ).
3. Transfer diluted sample from Step 1 into the bottom of each well.
4. Centrifuge for 5 min at 3,500 rpm in a centrifuge.
5. Add
100 µl Dye Wash buffer to each well on the plate.
6. Centrifuge for 20 min at 3,500 rpm in a centrifuge.
7. Add
20-30 µl of sequencing loading buffer or deionized water into
central of the membrane. Pipet up and down 5-10 times to elute the
sequencing product.
8. Transfer eluted products to a new 96 well
0.35ml collection plate.
Material Safety Data Sheet
1
96-Well SEQ Dye Clean Up Kit
Authorization date: 1/1/2006 Revision date: 1/1/2006
Print Date: 10/4/2006 Version: 1
1. IDENTIFICATION OF THE SUBSTANCE/PREPARATION AND THE COMPANY
/ UNDERTAKING
Commercial Product name:
96-Well SEQ Dye Clean Up Kit
Product number: GA-DCP02, GA-DCP04, GADCP10
Intended use: Biochemical kit
Supplie
r: Pacific Image Electronics Co., Ltd.
10F-1, No. 81, Hsin Tai Wu Rd., Sec. 1,
Hsi Chih, Taipei, Taiwan 221
Emergency telephone number:
+886 2 2698 0369
2. CHEMICAL CHARACTERISATION
Component 1:
Dye wash buffer: 1 Piece (s)
Ingredients 1-2.5% sodium Dodecyl sulfate; CASNo.
00151-21-3
3. HAZARDS IDENTIFICALTION
******************** EMERGENCY OVERVIEW **************
Component 1 – Occupational exposure presents little or no health
hazard.
*******************************************************
Potential Health Effects:
Eye: Can cause moderate irritation, tearing and reddening, but not
likely to permanently injure eye tissue. Can cause severe
irritation. Eye contact may result in corneal injury. Symptoms
may include discomfort or pain, excess blinking and tear
production, with marked redness and swelling of the
conjunctiva. Temporary vision impairment (cloudy or blurred
vision) is possible.
Skin: Can cause moderate skin irritation, defatting, and dermatitis.
Material Safety Data Sheet
2
Not likely to cause permanent damage. May cause allergic
skin reaction.
Inhalation: Can cause minor respiratory irritation, dizziness,
weakness, fatigue, nausea, and headache. Can be
corrosive to the respiratory tract causing severe irritation
and tissue damage. If liquid mists are breathed into the
lungs, they may be rapidly absorbed through the lungs
and injure specific target organs. No toxicity expected
from inhalation.
Ingestion: Mildly irritating to mouth, throat, and stomach. Can cause
abdominal discomfort. Harmful if swallowed. May cause
systemic poisoning. Corrosive to tissue. Can cause severe
and permanent damage to mouth, throat, and stomach.
Aspiration may lead to lung damage. Ingestion of this
product may result in central nervous system effects
including headache, sleepiness, dizziness, slurred speech
and blurred vision. Harmful if swallowed. May cause
systemic poisoning.
Chronic: No data on cancer. Contains a substance(s) that is a
possible reproductive system hazard based on high dose
tests with laboratory animals.
4. FIRST AID MEASURES
Eye: Flush eyes with plenty of water for at least 20 minutes
retracting eyelids often. Tilt the head to prevent chemical from
transferring to the uncontaminated eye. Remove contact
lenses, clean before re-use. Get immediate medical attention.
Skin: Wash with soap and water. Remove contaminated clothing
and launder immediately, and discard contaminated leather
goods, and wash before re-use. Get medical attention
immediately if irritation develops or persists.
Inhalation: Remove to fresh air. If breathing is difficult, have a
trained individual administer oxygen. If not breathing,
give artificial respiration and have a trained individual
administer oxygen. Get medical attention immediately.
Ingestion:
Complent 1 – Give plenty of water, if conscious. If
Material Safety Data Sheet
3
vomiting occurs naturally, wash mouth out. Be
prepared to induce vomiting upon a physician's advice.
Obtain medical attention if symptoms develop.
Note To Physician: Treat symptomatically.
5. FIRE FIGHTING MEASURE
Flashpoint Deg C: Not available.
Upper Flammable Limit %: Not available.
Lower Flammable Limit %: Not available.
Autoignition Temperature Deg C: Not available.
Extinguishing Media:
Complent 1, 4 and 5 – Use means
appropriate for surrounding materials.
Firefighting Techniques/Equipment: Do not enter fire area without
proper protection including selfcontained
breathing apparatus
and full protective equipment.
Fight fire from a safe distance
and a protected location due to
the potential of hazardous
vapors and decomposition
products.
Hazardous Combustion Products: Includes carbon dioxide, carbon
monoxide, and dense smoke.
6. ACCIDENTAL RELEASE MEASURES
Accidental releases may be subject to special reporting
requirements and other regulatory mandates. Refer to Section 8 for
personal protection equipment recommendations.
Spill Cleanup:
Complent 1, 4 and 5 – Absorb spill. Common
absorbent materials should be effective. Deposit in
appropriate containers for removal and disposal.
Material Safety Data Sheet
4
7. HANDLING AND STORAGE
Storage of some materials is regulated by federal, state, and/or
local laws.
Storage Pressure: Ambient
Handling Procedures:
Complent 1 – Keep closed or covered when
not in use.
Storage Procedures: Suitable for most general chemical storage
areas.
8. EXPOSURE CONTROLS, PERSONAL PROTECTION
Exposure Limits:
Component OSHA PEL (ppm) AGCIH TWA (ppm)
SODIUM DODECYL SULFATE Not established. Not established.
GUANIDINE HCL Not established. Not established.
Engineering Controls: Local exhaust ventilation or other engineering
controls are normally required when
handling or using this product to avoid
overexposure.
Personal Protective Equipment:
Eye: An eye wash station must be available where this product is
used. Wear chemical goggles.
Skin: Avoid skin contact by wearing chemically resistant gloves, an
apron and other protective equipment depending upon
conditions of use. Inspect gloves for chemical break-through
and replace at regular intervals. Clean protective equipment
regularly. Wash hands and other exposed areas with mild soap
and water before eating, drinking, and when leaving work.
Have a safety shower available.
Respiratory: A respiratory protection program that meets OSHA's
29 CFR 1910.134 and ANSI Z88.2 requirements must
be followed whenever workplace conditions warrant a
respirator's use.
Material Safety Data Sheet
5
9. PHYSICAL AND CHEMICAL PROPERTIES
Appearance/physical state: Liquid solution / suspension
Order: No odor.
Specific Gravity/Density: Not established.
Octanol/water Partition Coeff: Not established.
Volatiles: Not established.
Evaporation Rate: Not established.
Viscosity: Not established.
Infection: No Infection
10. STABILITY AND REACTIVITY
Stability: Stable under normal conditions.
Conditions to Avoid: Strong oxidizing agents. High temperatures.
Strong acids.
Hazardous Decomposition Products: None known.
Hazardous Polymerization: Hazardous polymerization will not occur.
11. TOXICOLOGICAL INFORMATION
Acute Toxicity
(Rat):
Dermal/Skin: Sodium Dodecyl Sulfate: Dermal LD50 >10 G/KG
Inhalation/Respiratory: GUANIDINE: 475 MG/KG 3250 MG/KG
Oral/Ingestion: Sodium dodecyl sulfate: Oral LD50 = 1288 MG/KG
Target Organs: Kidneys. Central Nervous System.
Carcinogenicity:
NTP: Not tested.
IARC: Not listed.
OSHA: Not regulated.
Other Toxicological Information
Contains a substance(s) that is a possible reproductive system
hazard based on high dose tests with laboratory animals.
12. Ecological Information
Material Safety Data Sheet
6
Ecotoxicological Information: No ecological information available.
Environmental Fate (Degradation,
Transformation, and Persistence):
Bioconcentration is not expected to
occur.
13. DISPOSAL CONSIDERATIONS
Regulatory Information: Not applicable.
Disposal Method: Clean up and dispose of waste in accordance with
all federal, state, and or local environmental.
14. TRANSPORT INFORMATION
NOT RESTRICTED ACCORDING TO IATA REGULATION.
Proper Shipping Name: Not Determined.
Subsidiary Hazards:-
15. OTHER INFORMATION
WHMIS Rating 0-4:
FIRE:0.
HEALTH: 2.
REACTIVITY: 0
Abbreviations
N/A - Data is not applicable or not available
SARA - Superfund and Reauthorization Act
HMIS - Hazard Material Information System
WHMIS - Workplace Hazard Materials Information System
NTP - National Toxicology Program
OSHA - Occupational Health and Safety Administration
IARC - International Agency for Research on Cancer
PROP 65 - California Safe Drinking Water and Toxic Enforcement Act
of 1986
EINECS - European Inventory of Existing Commercial
Chemical Substances