Category NameThyroid MarkersMethodELISAPrincipleELISAControls Standards0,1.5,3.2,6.5,15.0,20.0 pg/mlSensitivity0.05pg/mlSample50 µlRuntime75 minWavelength450 nmSafetyPlease refer to MSDSShelflife18 Months
rev.06
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REFERENCES
1. Agharanya JC. Clinical usefulness of ELISA technique in the assessment of thyroid function. West Afr J Med
1990;9(4):258-63.
2. Frank JE; Faix JE; Hermos RJ; Mullaney DM; Rojan DA; Mitchell ML; Klein RZ Thyroid function in very low birth
weight infants: effects on neonatal hypothyroidism screening. J Pediatr 1996;128(4):548-54.
3. Shimada T; Higashi K; Umeda T; Sato T. Thyroid functions in patients with various chronic liver diseases.
Endocrinol Jpn 1988;35(3):357-69.
4. Thakur C; Saikia TC; Yadav RN. Total serum levels of triiodothyronine (T3) thyroxine (T4) and thyrotropine (TSH)
in school going children of Dibrugarh district: an endemic goitre region of Assam. Indian J Physiol Pharmacol
1997;41(2):167-70.
THYROXINE (T4) ELISA
Catalog No. T4105T (96 tests)
INTENDED USE
The CBI T4 ELISA kit is used for the quantitative measurement of total Thyroxine (T4) in human serum or plasma.
SUMMARY AND EXPLANATION
T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in
hypothyroid patients and is increased in hyperthyroid patients. The level of T4 is normal in Euthyroid individuals.
PRINCIPLE OF THE TEST
The CBI T4 is a solid phases competitive ELISA. The samples, assay buffer and T4 enzyme conjugate are added to the
wells coated with anti-T4 monoclonal antibody. T4 in the patient’s serum competes with a T4 enzyme (HRP) conjugate
for binding sites. Unbound T4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the
substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is
prepared relating color intensity to the concentration of the T4.
MATERIALS PROVIDED 96 tests
1. Microwell coated with T4 MAb 12x8x1
2. T4 Standard: 6 vials ( ready to use) 1.0ml
3. Conjugate Buffer 12ml
4. TMB Substrate: 1 bottle (ready to use) 12ml
5. Stop Solution: 1 bottle (ready to use) 12ml
6. T4 Enzyme (HRP) Conjugate concentrate: 1 vial 1.5ml
7. 20X Wash concentrate: 1 bottle 25ml
MATERIALS NOT PROVIDED
1. Distilled or deionized water
2. Precision pipettes
3. Disposable pipette tips
4. ELISA reader capable of reading absorbance at 450nm
5. Absorbance paper or paper towel
6. Graph paper
STORAGE AND STABILITY
1. Store the kit at 2 - 8 ° C.
2. Keep microwells sealed in a dry bag with desiccants.
3. The reagents are stable until expiration of the kit.
4. Do not expose test reagents to heat, sun, or strong light.
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