Product Specification

Description                            IgG fraction of polyclonal rabbit antiserum to 3-phosphoglyceric phosphokinase from baker’s yeast

Product code                          NE130/7S

Biological origin                     Sheep

Physical form                         Purified hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative                           No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Immunogen                            3-Phosphoglyceric phosphokinase isolated and purified from baker’s yeast. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Purification                            The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.

Identity & Specificity           The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.

Cross-reactivity                     Cross-reactivities against enzymes of other sources may occur but have not been determined.

Physicochemical                    IgG protein concentration 10 mg/ml. No foreign proteins added.

characteristics

Intended use                         This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.

Directions for use                  Working dilutions in non-precipitating antibody-binding techniques may vary widely, but may be between 1/1,000 and 1/30,000.

Handling                                The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by addi ng 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Packing                                  Vial with 10 mg IgG (7S) fraction, lyophilised from phosphate buffered saline.

Storage / shelf life                  Lyophilised at +4°C                            at least 10 years

reconstituted at or below -20°C          3-5 years

reconstituted at +4°C                          7 days

Caution                                  This product should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro laboratory research purposes only.