Ready-to-go pre-mix with red dye
Datasheets
BioMix™ Red
|
Cat No. |
Size |
Conc |
|
BIO-25005 |
1.5mM |
100 Reactions |
|
BIO-25006 |
1.5mM |
500 Reactions |
Features
• Convenient pre-mixed, pre-optimized 2x solution
• Reduced risk of contamination
• Dramatically decreases the time required for reaction set-up
• Reproducible results
• Direct gel loading
Applications
• Routine PCR applications
• Products suitable for TA cloning
• High throughput
Description
BioMix™ Red is a complete ready-to-use 2x reaction mix containing an ultra-stable Taq DNA polymerase. It contains an additional inert red dye that permits easy visualization and direct loading onto a gel. There is no need to add loading buffer as the mix is of sufficiently high density to sink to the bottom of the gel.
BioMix Red has been developed to perform PCR assays of many common genomic and cDNA templates; the user has simply to add water, template and primers. It dramatically reduces the time required to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured, by reducing the number of pipetting steps that can lead to errors.
BioMix Red has been optimized for a wide variety of templates, however an additional 50mM of MgCl2 solution is included should any fine adjustments be required.
BioMix Red Protocol
Reaction Conditions (For a 50µl reaction)
|
Biomix Red |
25µl |
|
Template and Primers |
as required |
|
Water (ddH2O) |
up to 50µl |
Denature: 94-96°C
Elongate: 70-72°C (allowing 15-30 seconds/kb)
For optimal resolution of PCR products, we recommend the use of Tris-Acetate EDTA (TAE) buffer for gel preparation and electrophoresis.
An additional tube of 50mM MgCl2 is provided should any fine adjustments be necessary. The table below shows the volume of MgCl2 that must be added to a 50µl final reaction volume to achieve the desired final concentration.
|
Final MgCl2 Required |
MgCl2 to be added |
|
2.0mM |
0µl |
|
2.5mM |
0.5µl |
|
3.0mM |
1µl |
This data is intended for use as a guide only; conditions will vary from reaction to reaction and may need optimization.
Product Specifications
Batch details:
Batch No: See vial
Cat No: See vial
Components
|
BioMix Red |
100 Reactions |
500 Reactions |
|
BioMix Red |
2 x 1.25ml |
10 x 1.25ml |
|
50mM MgCl2 Solution |
1.2ml |
1.2ml |
Reagent Specifications:
MgCl2 Stock Solution: 50mM MgCl2
Storage Conditions:
BioMix Red can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.
Shipping Conditions:
On Dry Ice or Blue Ice
Associated Products
|
Product Name |
Pack Size |
Cat No |
|
HyperLadder I |
200 Lanes |
BIO-33025 |
|
Agarose |
100g |
BIO-41026 |
|
Agarose Tablets |
150g |
BIO-41028 |
|
SureClean Plus |
1 x 5ml |
BIO-37047 |
Product Citations:
1. Hancock, M.K., et al. The Journal of Biological Chemistry 277(49), 47205-47212 (2002).
2. Prado, M., et al. Electrophoresis 26(15), 2918-2926 (2005).
3. Reddy, S.T., et al. J. Biol. Chem 279(37), 38658-38667 (2004).
Notes
1. This product insert is a declaration of analysis at the time of manufacture.
2. Research Use Only.
|