Datasheets

MangoMix

 Features

• High throughput and cost-effective solution

• Direct gel loading

• Easy visual recognition

• Convenient pre-mixed, pre-optimized 2x solutions

• Reduced risk of contamination

• Dramatically decreases the time required for reaction set-up

Applications

• High throughput applications

• Suited to a wide range of PCR assays

• Products suitable for TA cloning

Description

MangoMix is a complete ready-to-use 2x pre-optimized reaction mix containing MangoTaq™ DNA Polymerase, dNTPs, red and orange reference dyes and Mg²⁺. MangoMix enables users to perform PCR assays of most common genomic and cDNA templates, simply requiring the addition of water, template and primers to perform the assays. MangoMix dramatically reduces the time required to set up reactions, thereby minimizing the risk of contamination.

The red and orange dyes facilitate easy recognition and separate during electrophoresis to provide quick reference points for monitoring the mobility of the DNA samples in the gel. There is no need for separate gel-loading buffer. The presence of the dyes has no effect on routine enzymatic manipulations, although rare exceptions may exist.

MangoMix Protocol

Reaction Conditions (For a 50µl reaction)

MangoMix                              25µl

Template and Primers             as required

Water (ddH2O)                      up to 50µl

Denature: 94-96°C

Elongate: 70-72°C (allowing 15-30 seconds/kb)

For optimal resolution of PCR products, we recommend the use of Tris-Acetate EDTA (TAE) buffer for gel preparation and electrophoresis.

MangoMix is supplied with 4mM MgCl₂, giving a final reaction concentration of 2mM, which in the presence of the reaction additives and stabilizers provides excellent performance and specificity.

MangoMix has been optimized for a wide variety of templates; however an additional 50mM of MgCl₂ solution is included in case any fine adjustments are required.

 This data is intended for use as a guide only; conditions will vary from reaction to reaction and may need optimization.

Product Specifications

Batch details:

Batch No:        See vial

Cat No:           See vial

Components

Reagent Specifications:

MgCl2 Stock Solution: 50mM MgCl₂

Storage Conditions:

MangoMix can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Avoid repeat freeze/thaw cycles.

Shipping Conditions:

On Dry Ice or Blue Ice

Associated Products: