Datasheets
Features
- Quick isolation of high-quality RNA
- Ready-to-use solution for a wide variety cells and tissues
- Cost-effective
- Convenient 1hr protocol
- Performs well with large or small amounts of tissue or cells
Applications
- Isolation of high quality RNA from diverse biological material, including animal and plant tissues rich in polysaccharides and proteoglycans
- Purified RNA is ideal for any downstream application such as RT-PCR or in vitro translation
Description
TRIsure is a ready-to-use reagent for the isolation of Total RNA from cells and tissues. TRIsure maintains the integrity of the extracted RNA, while disrupting cells and subsequently dissolving cell components. The reagent combines a blend of phenol and other components.
The biological sample is homogenized or lysed in TRIsure and then separated into organic and aqueous phases. The RNA remains in the aqueous phase and is subsequently recovered by precipitation with isopropyl alcohol.
The isolated RNA is suitable for any downstream application such as RT-PCR, hybridization assays, or in vitro translation. 1ml of TRIsure is sufficient to isolate Total RNA from 1 x 107 cells or 100mg of tissue.
From 1mg of tissue or 1 x 106 of cultured cells, the expected yield of RNA is:
- 2-5µg from mouse kidney (tissue)
- 5-10µg from mouse liver (tissue)
- 8-15µg from epithelial cells (cultured cells)
- 20-25µg from cell fibroblasts (cultured cells)
Storage Conditions
TRIsure can be stored for 12 months at +4°C.
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