Size : 200µmol

Final concentration : 100mM total

Presentation 4*500µL

Product:
dNTP Mix, ready-to-use. Available in 10mM, 40mM and 100mM concentrations.

Features
        • Convenient, pre-optimized and pre-mixed
        • Ultra-pure: >99% trisphosphate by HPLC
        • Extended shelf-life of 24 months at -20°C
        • Free from PCR inhibitors
        • DNase, RNase and Nickase free
        • Manufactured by Bioline in a purpose-built facility

Applications
Suitable for a wide variety of applications such as:
        • Standard and long range PCR assays
        • cDNA synthesis
        • qPCR
        • Microarrays
        • DNA sequencing
        • DHPLC
        • Labeling

Description
A ready-to-use molecular grade dNTP Mix containing dATP, dCTP, dGTP and dTTP at pH 7.5 as Lithium salts in purified water. The mix is designed to save hands-on time for researchers and minimize the possibility of contamination. For use in DNA polymerization reactions, DNA labeling and sequencing processes. Dependable PCR grade.
Improved Stability and Extended Shelf-Life All Bioline dNTPs are supplied as Lithium salts in purified water at pH 7.5. Lithium salts have greater resistance to repeated freezing and thawing cycles than Sodium salts, and Lithium salt dNTP preparations remain sterile over the entire shelf-life due to the bacteriostatic activity of Lithium towards various microorganisms.

dNTP Mix Reaction Guidelines
100mM Mix contains 25 mM of each dNTP
Reaction Volume      Master Mix        Reactions
50µl                          0.5µl                1000
40mM Mix contains 10 mM of each dNTP
Reaction Volume      Master Mix        Reactions
50µl                         1.25µl               400
10mM Mix contains 2.5 mM of each dNTP
Reaction Volume       Master Mix      Reactions
50µl                          5.0µl               200
This is a guide only, for long-range applications adjust accordingly.

Product Specifications
A mix of dATP, dCTP, dGTP, and dTTP, (pH 7.5).

Storage Conditions:
dNTP Mix can be stored for 24 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended.

Shipping Conditions:
On Dry Ice or Blue Ice

Typical Analysis:
Lithium salts, >99% deoxynucleoside triphosphates (HPLC, area %), <1% deoxynucleoside monophosphates and deoxynucleoside diphosphates.

Purity:
dNTPs are >99% pure by HPLC and are free of DNase, RNase, Protease, phosphatase and nicking activity.
dNTP Characteristics:
dNTPs       Mol. Wt      Molar Extinction(1µmol)
dATP         514.9                 15.4 (A259)
dGTP         530.9                 13.7 (A252)
dCTP         490.9                   9.1 (A272)
dTTP          505.9                   9.6 (A267)

Product Citations:
1. Tabone, T., et al. Nucleic Acids Research, 34(6), e45, 2006.
2. Dean, Y.D., et al. J. Biol. Chem. 275(44), 34382-34392, 2000.
3. Lloyd, R.E., et al. Genetics, 172, 2515-2527, 2006.