Gravity-flow columns for purification of up to 500 ug transfection grade plasmid DNA

• Purity equivalent to that obtained by 2 x CsCl-gradient centrifugation
• Reproducible yields of transfection grade plasmid DNA
• No ethidium bromide, phenol, chloroform, or CsCl
• Cost-effective preparations

Principle

The unique anion-exchange resin in QIAGEN-tips is developed exclusively for the purification of nucleic acids. Its exceptional separation properties result in DNA purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation.Pre-packed QIAGEN-tips operate by gravity flow and never run dry, minimizing the hands-on time required for plasmid preparation. The entire QIAGEN plasmid purification system avoids the use of toxic substances such as phenol, chloroform, ethidium bromide, and CsCl, minimizing hazard both to the user and the environment.